A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes

Ghennady V. Gushchin; Marina I. Gushchin; Nicholas Gerber; R. Thomas Boyd

doi:10.1006/bbrc.1999.0200

A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes

Ghennady V. Gushchin
, Marina I. Gushchin
, Nicholas Gerber
, R. Thomas Boyd

Neuroscience

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

PCR with several pairs of primers facilitates screening for new isoenzymes among highly homologous cytochrome P450s (CYPs). Combinations of two pairs of primers, which amplify N- and C-terminal coding sequences of either CYP3A1/CYP3A23 or CYP3A2 detected the presence of a previously unrecognized CYP3A in enterocyte microsomes isolated from rats. PCR, Northern blot, and immunoblotting with specific antibodies indicated that this isoenzyme is clearly distinguishable from CYP3A1, 3A23 or 3A2. Sequencing of a 285 bp coding fragment of this gene revealed 97% similarity with rat olfactory CYP3A9 (P450olf3).

Original language	English
Pages (from-to)	394-398
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	255
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1999.0200
State	Published - Feb 16 1999

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title = "A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes",

abstract = "PCR with several pairs of primers facilitates screening for new isoenzymes among highly homologous cytochrome P450s (CYPs). Combinations of two pairs of primers, which amplify N- and C-terminal coding sequences of either CYP3A1/CYP3A23 or CYP3A2 detected the presence of a previously unrecognized CYP3A in enterocyte microsomes isolated from rats. PCR, Northern blot, and immunoblotting with specific antibodies indicated that this isoenzyme is clearly distinguishable from CYP3A1, 3A23 or 3A2. Sequencing of a 285 bp coding fragment of this gene revealed 97\% similarity with rat olfactory CYP3A9 (P450olf3).",

author = "Gushchin, \{Ghennady V.\} and Gushchin, \{Marina I.\} and Nicholas Gerber and Boyd, \{R. Thomas\}",

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T1 - A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes

AU - Gushchin, Ghennady V.

AU - Gushchin, Marina I.

AU - Gerber, Nicholas

AU - Boyd, R. Thomas

PY - 1999/2/16

Y1 - 1999/2/16

N2 - PCR with several pairs of primers facilitates screening for new isoenzymes among highly homologous cytochrome P450s (CYPs). Combinations of two pairs of primers, which amplify N- and C-terminal coding sequences of either CYP3A1/CYP3A23 or CYP3A2 detected the presence of a previously unrecognized CYP3A in enterocyte microsomes isolated from rats. PCR, Northern blot, and immunoblotting with specific antibodies indicated that this isoenzyme is clearly distinguishable from CYP3A1, 3A23 or 3A2. Sequencing of a 285 bp coding fragment of this gene revealed 97% similarity with rat olfactory CYP3A9 (P450olf3).

AB - PCR with several pairs of primers facilitates screening for new isoenzymes among highly homologous cytochrome P450s (CYPs). Combinations of two pairs of primers, which amplify N- and C-terminal coding sequences of either CYP3A1/CYP3A23 or CYP3A2 detected the presence of a previously unrecognized CYP3A in enterocyte microsomes isolated from rats. PCR, Northern blot, and immunoblotting with specific antibodies indicated that this isoenzyme is clearly distinguishable from CYP3A1, 3A23 or 3A2. Sequencing of a 285 bp coding fragment of this gene revealed 97% similarity with rat olfactory CYP3A9 (P450olf3).

UR - https://www.scopus.com/pages/publications/0033574047

U2 - 10.1006/bbrc.1999.0200

DO - 10.1006/bbrc.1999.0200

M3 - Article

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AN - SCOPUS:0033574047

SN - 0006-291X

VL - 255

SP - 394

EP - 398

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes

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