TY - JOUR
T1 - ACUTE MYELOGENOUS LEUKAEMIA WITH C-MYC AMPLIFICATION AND DOUBLE MINUTE CHROMOSOMES
AU - Alitalo, Kari
AU - Winqvist, Robert
AU - Keski-Oja, Jorma
AU - Ilvonen, Mirja
AU - Saksela, Kalle
AU - Alitalo, Riitta
AU - Laiho, Marikki
AU - Knuutila, Sakari
AU - De La Chapelle, Albert
N1 - Funding Information:
We thank Mervi Laukkanen for typing the manuscript. This work was supported by grants from the Cancer Societies of Finland, the Academy of Finland, the Sigrid Juselius Foundation, and the Finska Läkaresällskapet.
PY - 1985/11/9
Y1 - 1985/11/9
N2 - Cytogenetic analysis of bone-marrow cells from a woman with preleukaemia showed numerous mitoses with trisomy 4 and double minute chromosomes. These abnormalities were later seen in blood cells during subsequent acute myeloid leukaemia (AML). Complete remission was achieved with three courses of doxorubicin, cytosine arabinoside, and prednisone. A further clonal abnormality, trisomy 6, was seen in leukaemic cells after the first relapse. Analyses of total DNA from the peripheral-blood cells during relapse showed that the c-myconcogene was amplified about 30-fold in the leukaemic cells. The N-myc, c-mos, and c-myb oncogenes showed only single-copy signals. On average about two copies of c-myc resided on each dmin chromosome. The finding of amplification of a cellular oncogene (c-myc) in fresh AML cells containing double minute chromosomes suggests that clonal evolution of some leukaemia cell populations may involve selection for increased dosage of oncogenes.
AB - Cytogenetic analysis of bone-marrow cells from a woman with preleukaemia showed numerous mitoses with trisomy 4 and double minute chromosomes. These abnormalities were later seen in blood cells during subsequent acute myeloid leukaemia (AML). Complete remission was achieved with three courses of doxorubicin, cytosine arabinoside, and prednisone. A further clonal abnormality, trisomy 6, was seen in leukaemic cells after the first relapse. Analyses of total DNA from the peripheral-blood cells during relapse showed that the c-myconcogene was amplified about 30-fold in the leukaemic cells. The N-myc, c-mos, and c-myb oncogenes showed only single-copy signals. On average about two copies of c-myc resided on each dmin chromosome. The finding of amplification of a cellular oncogene (c-myc) in fresh AML cells containing double minute chromosomes suggests that clonal evolution of some leukaemia cell populations may involve selection for increased dosage of oncogenes.
UR - https://www.scopus.com/pages/publications/0022403193
U2 - 10.1016/S0140-6736(85)90907-9
DO - 10.1016/S0140-6736(85)90907-9
M3 - Article
C2 - 2865517
AN - SCOPUS:0022403193
SN - 0140-6736
VL - 326
SP - 1035
EP - 1039
JO - The Lancet
JF - The Lancet
IS - 8463
ER -