Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)

Evgeny Vinogradov; Wayne J. Conlan; John S. Gunn; Malcolm B. Perry

doi:10.1016/j.carres.2003.12.013

Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)

Evgeny Vinogradov
, Wayne J. Conlan
, John S. Gunn
, Malcolm B. Perry

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, ¹H and ¹³C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D- galacturonamide (D-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure:→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→4) -α-D-GalNAcAN-(1→3)-α-D-QuiNAc4NAc-(1→ With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common →4)-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→ disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species. Crown

Original language	English
Pages (from-to)	649-654
Number of pages	6
Journal	Carbohydrate Research
Volume	339
Issue number	3
DOIs	https://doi.org/10.1016/j.carres.2003.12.013
State	Published - Feb 25 2004

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title = "Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)",

abstract = "Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, 1H and 13C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D- galacturonamide (D-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure:→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→4) -α-D-GalNAcAN-(1→3)-α-D-QuiNAc4NAc-(1→ With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common →4)-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→ disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species. Crown",

author = "Evgeny Vinogradov and Conlan, \{Wayne J.\} and Gunn, \{John S.\} and Perry, \{Malcolm B.\}",

note = "Funding Information: We thank the National Institutes of Health, USA for their support of this work through grant R01-AI 48474.",

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doi = "10.1016/j.carres.2003.12.013",

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T1 - Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)

AU - Vinogradov, Evgeny

AU - Conlan, Wayne J.

AU - Gunn, John S.

AU - Perry, Malcolm B.

N1 - Funding Information: We thank the National Institutes of Health, USA for their support of this work through grant R01-AI 48474.

PY - 2004/2/25

Y1 - 2004/2/25

N2 - Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, 1H and 13C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D- galacturonamide (D-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure:→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→4) -α-D-GalNAcAN-(1→3)-α-D-QuiNAc4NAc-(1→ With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common →4)-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→ disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species. Crown

AB - Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, 1H and 13C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D- galacturonamide (D-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure:→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→4) -α-D-GalNAcAN-(1→3)-α-D-QuiNAc4NAc-(1→ With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common →4)-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→ disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species. Crown

UR - https://www.scopus.com/pages/publications/0842265909

U2 - 10.1016/j.carres.2003.12.013

DO - 10.1016/j.carres.2003.12.013

M3 - Article

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SN - 0008-6215

VL - 339

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EP - 654

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 3

ER -

Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)

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