TY - JOUR
T1 - Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12
AU - Takayama, Shinichi
AU - Kochel, Kristine
AU - Irie, Shinji
AU - Inazawa, Johji
AU - Abe, Tatsuo
AU - Sato, Takaaki
AU - Druck, Teresa
AU - Huebner, Kay
AU - Reed, John C.
N1 - Funding Information:
We acknowledge the support of the NCI (CA-67329) (CA-51083), Louie Coffman and the RAAM Team for a fellowship grant to S.T., the Leukemia Society of America for a Scholar Award to J.C.R., and the Japanese Ministry of Education for a grant to T.A. and J.I.
PY - 1996/8/1
Y1 - 1996/8/1
N2 - cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2- terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein (~34 kDa) compared to mouse. PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death.
AB - cDNAs encoding the human homolog of BAG1, a Bcl-2-binding protein with anti-apoptotic function, were cloned. DNA sequence analysis of human BAG1 cDNAs predicts a protein with an additional 55 amino acids at its NH2- terminus compared to the mouse protein. Immunoblot assays using monoclonal antibodies raised against bacterially produced h-BAG1 protein confirmed the larger size of the human protein (~34 kDa) compared to mouse. PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers localized the gene to human chromosome 9. Cosmid clones of h-BAG1 were obtained and used for fluorescence in situ hybridization analysis of normal metaphase chromosomes, thus localizing h-BAG1 to 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death.
UR - https://www.scopus.com/pages/publications/0030219848
U2 - 10.1006/geno.1996.0389
DO - 10.1006/geno.1996.0389
M3 - Article
C2 - 8812483
AN - SCOPUS:0030219848
SN - 0888-7543
VL - 35
SP - 494
EP - 498
JO - Genomics
JF - Genomics
IS - 3
ER -