Demonstration of a cellular inhibitor of Epstein-Barr and cytomegalovirus synthesis

Ronald Glaser; Joseph Zimmerman; Stephen St. Jeor; Fred Rapp

doi:10.1016/0042-6822(75)90103-8

Demonstration of a cellular inhibitor of Epstein-Barr and cytomegalovirus synthesis

Ronald Glaser
, Joseph Zimmerman
, Stephen St. Jeor
, Fred Rapp

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A particle free extract prepared from human D98 cells was tested for inhibitory activity against the Epstein-Barr Virus (EBV) and cytomegalovirus (CMV). The number of permissive human cells positive for EBV-specific antigens after infection with virus decreased by more than 90% when the cells were treated with D98 extract and poly-l-ornithine. Synthesis of CMV DNA was depressed in virus infected cells in the presence of the inhibitor. Replication of infectious CMV was reduced by approximately 99.9%. The data suggest that the D98 extract specifically depresses EBV and CMV replication.

Original language	English
Pages (from-to)	289-292
Number of pages	4
Journal	Virology
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(75)90103-8
State	Published - Mar 1975

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@article{18883a73735b4991b6d004ff93f6b400,

title = "Demonstration of a cellular inhibitor of Epstein-Barr and cytomegalovirus synthesis",

abstract = "A particle free extract prepared from human D98 cells was tested for inhibitory activity against the Epstein-Barr Virus (EBV) and cytomegalovirus (CMV). The number of permissive human cells positive for EBV-specific antigens after infection with virus decreased by more than 90\% when the cells were treated with D98 extract and poly-l-ornithine. Synthesis of CMV DNA was depressed in virus infected cells in the presence of the inhibitor. Replication of infectious CMV was reduced by approximately 99.9\%. The data suggest that the D98 extract specifically depresses EBV and CMV replication.",

author = "Ronald Glaser and Joseph Zimmerman and \{St. Jeor\}, Stephen and Fred Rapp",

note = "Funding Information: The authors thank Ross Farrugia and Martin Nau for excellent technical assistance. The authors also thank Dr. Werner Henle for the EBV-specific antiserum. This study was conducted, in part, under Contract No. 70-2024 within the Virus Cancer Program of the National Cancer Institute and by Public Health Service Research Grant No. CA-15038-OlAl from the National Cancer Institute.",

year = "1975",

month = mar,

doi = "10.1016/0042-6822(75)90103-8",

language = "English",

volume = "64",

pages = "289--292",

journal = "Virology",

issn = "0042-6822",

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TY - JOUR

T1 - Demonstration of a cellular inhibitor of Epstein-Barr and cytomegalovirus synthesis

AU - Glaser, Ronald

AU - Zimmerman, Joseph

AU - St. Jeor, Stephen

AU - Rapp, Fred

N1 - Funding Information: The authors thank Ross Farrugia and Martin Nau for excellent technical assistance. The authors also thank Dr. Werner Henle for the EBV-specific antiserum. This study was conducted, in part, under Contract No. 70-2024 within the Virus Cancer Program of the National Cancer Institute and by Public Health Service Research Grant No. CA-15038-OlAl from the National Cancer Institute.

PY - 1975/3

Y1 - 1975/3

N2 - A particle free extract prepared from human D98 cells was tested for inhibitory activity against the Epstein-Barr Virus (EBV) and cytomegalovirus (CMV). The number of permissive human cells positive for EBV-specific antigens after infection with virus decreased by more than 90% when the cells were treated with D98 extract and poly-l-ornithine. Synthesis of CMV DNA was depressed in virus infected cells in the presence of the inhibitor. Replication of infectious CMV was reduced by approximately 99.9%. The data suggest that the D98 extract specifically depresses EBV and CMV replication.

AB - A particle free extract prepared from human D98 cells was tested for inhibitory activity against the Epstein-Barr Virus (EBV) and cytomegalovirus (CMV). The number of permissive human cells positive for EBV-specific antigens after infection with virus decreased by more than 90% when the cells were treated with D98 extract and poly-l-ornithine. Synthesis of CMV DNA was depressed in virus infected cells in the presence of the inhibitor. Replication of infectious CMV was reduced by approximately 99.9%. The data suggest that the D98 extract specifically depresses EBV and CMV replication.

UR - https://www.scopus.com/pages/publications/0016613436

U2 - 10.1016/0042-6822(75)90103-8

DO - 10.1016/0042-6822(75)90103-8

M3 - Article

C2 - 163544

AN - SCOPUS:0016613436

SN - 0042-6822

VL - 64

SP - 289

EP - 292

JO - Virology

JF - Virology

IS - 1

ER -

Demonstration of a cellular inhibitor of Epstein-Barr and cytomegalovirus synthesis

Abstract

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