Electroporation of siRNA to silence gene expression in primary NK cells

Prasad V. Phatarpekar; Dean A. Lee; Srinivas S. Somanchi

doi:10.1007/978-1-4939-3684-7_22

Electroporation of siRNA to silence gene expression in primary NK cells

Prasad V. Phatarpekar, Dean A. Lee, Srinivas S. Somanchi

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

8 Scopus citations

Abstract

Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection®. The technique yields effective silencing of the target gene without any off-target effects.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	267-276
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-3684-7_22
State	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1441
ISSN (Print)	1064-3745

Keywords

Electroporation
Gene silencing
Human primary NK cell
Nucleofection
ON-TARGET plus
SMART pool siRNA
siRNA

Access to Document

10.1007/978-1-4939-3684-7_22

Cite this

@inbook{ad932c831b8f425bb5facada12241f02,

title = "Electroporation of siRNA to silence gene expression in primary NK cells",

abstract = "Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection{\textregistered}. The technique yields effective silencing of the target gene without any off-target effects.",

keywords = "Electroporation, Gene silencing, Human primary NK cell, Nucleofection, ON-TARGET plus, SMART pool siRNA, siRNA",

author = "Phatarpekar, {Prasad V.} and Lee, {Dean A.} and Somanchi, {Srinivas S.}",

year = "2016",

doi = "10.1007/978-1-4939-3684-7_22",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "267--276",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Electroporation of siRNA to silence gene expression in primary NK cells

AU - Phatarpekar, Prasad V.

AU - Lee, Dean A.

AU - Somanchi, Srinivas S.

PY - 2016

Y1 - 2016

N2 - Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection®. The technique yields effective silencing of the target gene without any off-target effects.

AB - Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection®. The technique yields effective silencing of the target gene without any off-target effects.

KW - Electroporation

KW - Gene silencing

KW - Human primary NK cell

KW - Nucleofection

KW - ON-TARGET plus

KW - SMART pool siRNA

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=84979294975&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3684-7_22

DO - 10.1007/978-1-4939-3684-7_22

M3 - Chapter

C2 - 27177673

AN - SCOPUS:84979294975

T3 - Methods in Molecular Biology

SP - 267

EP - 276

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Electroporation of siRNA to silence gene expression in primary NK cells

Abstract

Publication series

Keywords

Access to Document

Other files and links

Fingerprint

Cite this