TY - JOUR
T1 - Environmental influences on fatty acid composition of membranes from autoimmune MRL lpr/lpr mice
AU - Hackshaw, Kevin V.
AU - Kunesh, Sarah L.
AU - Jackson, Nkenge A.
AU - Culpepper, Chantai
N1 - Funding Information:
The authors wish to thank Dr Jay Y. Westcott (University of Colorado Health Sciences Center) for measurement of 6-keto-PGFI~ and LTC4i n culture supernatants. This work was supported in part by grants from the following: The Ohio State University SEED Foundation and USPHS Grant K11 A101048-01 to Kevin Hackshaw. Nkenge A. Jackson is a recipient of the OSU Summer Research Opportunities Program (SROP) Scholarship. Chantal Culpepper is a recipient of the Samuel J. Roessler Memorial Research Award from The Ohio State University College of Medicine.
PY - 1996
Y1 - 1996
N2 - We analyzed fatty acid make up of cells and organs from autoimmune and immunologically normal mice to determine whether intrinsic differences in composition might be associated with an inflammatory phenotype. Macrophages (M∅) isolated from 4-6-week-old MRL lpr/lpr mice were cultured with phorbol ester (PMA), fibroblast growth factor-1 (FGF-1), fibroblast growth factor-2 (FGF-2) and medium control to determine whether these cell signals might induce membrane fatty acid changes. Individual phospholipid analysis showed 8.4- and 5.1-fold increases in phosphatidylcholine arachidonate (20:4) mole % over baseline values following culture with FGF-1 and FGF-2, respectively. Unfractionated analysis on kidney and liver extracts from 4-6 week MRL lpr/lpr, 16-20 week lpr and 12-20 week MRL ±/± mice demonstrated no significant intrastrain fatty acid differences. Higher levels of 20:4 in 4-6 week lpr mice were noted compared to 16-20 week lpr or +/+ mice in kidney, and liver samples (P < 0.05). It is possible that membrane changes precipitated by microenvironmental cytokine concentrations may contribute to the expression of autoimmune disease in this model.
AB - We analyzed fatty acid make up of cells and organs from autoimmune and immunologically normal mice to determine whether intrinsic differences in composition might be associated with an inflammatory phenotype. Macrophages (M∅) isolated from 4-6-week-old MRL lpr/lpr mice were cultured with phorbol ester (PMA), fibroblast growth factor-1 (FGF-1), fibroblast growth factor-2 (FGF-2) and medium control to determine whether these cell signals might induce membrane fatty acid changes. Individual phospholipid analysis showed 8.4- and 5.1-fold increases in phosphatidylcholine arachidonate (20:4) mole % over baseline values following culture with FGF-1 and FGF-2, respectively. Unfractionated analysis on kidney and liver extracts from 4-6 week MRL lpr/lpr, 16-20 week lpr and 12-20 week MRL ±/± mice demonstrated no significant intrastrain fatty acid differences. Higher levels of 20:4 in 4-6 week lpr mice were noted compared to 16-20 week lpr or +/+ mice in kidney, and liver samples (P < 0.05). It is possible that membrane changes precipitated by microenvironmental cytokine concentrations may contribute to the expression of autoimmune disease in this model.
UR - https://www.scopus.com/pages/publications/0029892891
U2 - 10.1016/S0952-3278(96)90063-0
DO - 10.1016/S0952-3278(96)90063-0
M3 - Article
C2 - 8804129
AN - SCOPUS:0029892891
SN - 0952-3278
VL - 54
SP - 297
EP - 302
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 4
ER -