Feasibility of a multimodal ¹⁸F-FDG-directed lymph node surgical excisional biopsy approach for appropriate diagnostic tissue sampling in patients with suspected lymphoma

Background: F-FDG PET/CT imaging is widely utilized in the clinical evaluation of patients with suspected or documented lymphoma. The aim was to describe our cumulative experience with a multimodal ¹⁸F-FDG-directed lymph node surgical excisional biopsy approach in patients with suspected lymphoma. Methods: Thirteen patients (mean age 51 (±16;22-76) years), with suspected new or suspected recurrent lymphoma suggested by ¹⁸F-FDG-avid lesions seen on prior diagnostic whole-body PET/CT imaging, were injected IV with ¹⁸F-FDG prior to undergoing same-day diagnostic lymph node surgical excisional biopsy in the operating room. Various ¹⁸F-FDG detection strategies were used on the day of surgery, including, (1) same-day pre-resection patient PET/CT; (2) intraoperative gamma probe assessment; (3) clinical scanner specimen PET/CT imaging of whole surgically excised tissue specimens; (4) specimen gamma well counts; and/or (5) same-day post-resection patient PET/CT. Results: Same-day ¹⁸F-FDG injection dose was 14.8 (±2.4;12.5-20.6) millicuries or 548 (±89;463-762) megabecquerels. Sites of ¹⁸F-FDG-avid lesions were 4 inguinal, 3 cervical, 3 abdominal/retroperitoneal, 2 axillary, and 1 gluteal region subcutaneous tissue. Same-day pre-resection patient PET/CT was performed on 6 patients. Intraoperative gamma probe assessment was performed on 13 patients. Clinical scanner PET/CT imaging of whole surgically excised tissue specimens was performed in 10 cases. Specimen gamma well counts were performed in 6 cases. Same-day post-resection patient PET/CT imaging was performed on 8 patients. Time from ¹⁸F-FDG injection to same-day pre-resection patient PET/CT, intraoperative gamma probe assessment, and same-day post-resection patient PET/CT were 76 (±8;64-84), 240 (±63;168-304), and 487 (±104;331-599) minutes, respectively. Time from ¹⁸F-FDG injection to clinical scanner PET/CT of whole surgically excised tissue specimens was 363 (±60;272-446) minutes. Time from ¹⁸F-FDG injection to specimen gamma well counts was 591 (±96;420-689) minutes. Intraoperative gamma probe assessment successfully identified ¹⁸F-FDG-avid lesions in 12/13 patients. Histopathologic evaluation confirmed lymphoma in 12/13 patients and benign disease in 1/13 patients. Conclusions: A multimodal approach to ¹⁸F-FDG-directed lymph node surgical excisional biopsy for suspected lymphoma is technically feasible for guiding appropriate diagnostic tissue sampling of lymph nodes seen as ¹⁸F-FDG-avid lesions on diagnostic ¹⁸F-FDG PET/CT imaging.