TY - JOUR
T1 - Heterogeneity of epstein-barr virus derived from a nasopharyngeal carcinoma that has transforming and lytic properties
AU - Sato, Hiroshi
AU - Takimoto, Toru
AU - Ogura, Hisashi
AU - Tanaka, Junji
AU - Hatano, Motoichi
AU - Glaser, Ronald
N1 - Funding Information:
I Received May 28, 1985; revised November 25, 1985; accepted December 23, 1985. 2 Supported in part by Public Health Service grants CA-16058 and CA-29066 from the Division of Extramural Activities, National Cancer Institute. S Department of Virology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, 920 Kanazawa, Japan. 4 Department of Medical Microbiology and Immunology, The Ohio State University College of Medicine, Columbus, OH 43210. 5 On leave from the Department of Otorhinolaryngology, School of Medicine, Kanazawa University. 6 Comprehensive Cancer Center, Columbus, OH 43210. 7 Address reprint requests to Dr. Glaser, Department of Medical Microbiology and Immunology, The Ohio State University College of Medicine, 333 West 10th Ave., Columbus, OH 43210.
PY - 1986/6
Y1 - 1986/6
N2 - The biological activities of Epstein-Barr virus (EBV) from a human epithelial hybrid cell line derived from a nasopharyngeal carcinoma (NPC-KT) were studied. low concentrations of virus from the NPC-KT cell line stimulated DNA synthesis of human cord blood lymphocytes (CBL) and induced CBL to form EBV nuclear antigen-positive continuous cell lines. The CBL exposed to higher doses of virus showed stimulated DNA synthesis 2 days after infection, followed by cell lysis. Virus from the NPC-KT cell line induced EBV-specific antigens in non producer Raji cells and reduced the colony-forming ability of the superinfected cells. The ratio of transforming activity to early antigeninducing ability was not constant during cell passage. The data obtained suggest that NPC-KT clone 1 cells are producing virus with cytotoxic and transforming properties.
AB - The biological activities of Epstein-Barr virus (EBV) from a human epithelial hybrid cell line derived from a nasopharyngeal carcinoma (NPC-KT) were studied. low concentrations of virus from the NPC-KT cell line stimulated DNA synthesis of human cord blood lymphocytes (CBL) and induced CBL to form EBV nuclear antigen-positive continuous cell lines. The CBL exposed to higher doses of virus showed stimulated DNA synthesis 2 days after infection, followed by cell lysis. Virus from the NPC-KT cell line induced EBV-specific antigens in non producer Raji cells and reduced the colony-forming ability of the superinfected cells. The ratio of transforming activity to early antigeninducing ability was not constant during cell passage. The data obtained suggest that NPC-KT clone 1 cells are producing virus with cytotoxic and transforming properties.
UR - https://www.scopus.com/pages/publications/0022592840
U2 - 10.1093/jnci/76.6.1019
DO - 10.1093/jnci/76.6.1019
M3 - Article
C2 - 3012175
AN - SCOPUS:0022592840
SN - 0027-8874
VL - 76
SP - 1019
EP - 1024
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -