Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein

Xiaoyu Niu; Jiayu Xu; Mingde Liu; Huolin Tu; Sara N. Koenig; Linda J. Saif; Daniel M. Jones; Qiuhong Wang

doi:10.1007/s00705-022-05621-5

Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein

Xiaoyu Niu, Jiayu Xu, Mingde Liu, Huolin Tu, Sara N. Koenig, Linda J. Saif, Daniel M. Jones, Qiuhong Wang

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Abstract

We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021.

Original language	English
Article number	5
Journal	Archives of Virology
Volume	168
Issue number	1
DOIs	https://doi.org/10.1007/s00705-022-05621-5
State	Published - Jan 2023

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title = "Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein",

abstract = "We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021.",

author = "Xiaoyu Niu and Jiayu Xu and Mingde Liu and Huolin Tu and Koenig, {Sara N.} and Saif, {Linda J.} and Jones, {Daniel M.} and Qiuhong Wang",

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AU - Niu, Xiaoyu

AU - Xu, Jiayu

AU - Liu, Mingde

AU - Tu, Huolin

AU - Koenig, Sara N.

AU - Saif, Linda J.

AU - Jones, Daniel M.

AU - Wang, Qiuhong

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AB - We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021.

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Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein

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