Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy

Marcelo de Souza Fernandes Pereira, Aarohi Thakkar, Dean Anthony Lee

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations

Abstract

The field of cellular immunotherapy for cancer has experienced exponential growth over the last decade. Several chimeric antigen receptor T cell products have already received FDA approval, which has stimulated growth and enthusiasm for other cellular therapies. Preclinical models are critical steps in the development of these products, and understanding their in vivo trafficking and persistence are critical components of their efficacy and toxicity analogous to volume of distribution and tissue penetration in small molecule therapeutics. Thus, well-established preclinical methodologies for following cells after adoptive transfer are important to understanding immune cell trafficking to, and persistence in, tumors or organs of interest. Here, we describe a quick and reliable method for labeling and in vivo tracking of immune cells adoptively transferred into small animal models by using in vivo fluorescent imaging.

Original languageEnglish
Title of host publicationCAR T cells
Subtitle of host publicationdevelopment, characterization and applications
EditorsSheila Spada, Lorenzo Galluzzi
PublisherAcademic Press Inc.
Pages163-170
Number of pages8
ISBN (Print)9780323855013
DOIs
StatePublished - Jan 2022

Publication series

NameMethods in Cell Biology
Volume167
ISSN (Print)0091-679X

Keywords

  • Adoptive transfer
  • CAR-T cells
  • DiR
  • Fluorescence imaging
  • IVIS
  • Immunotherapy
  • NK cells
  • Preclinical model
  • Systemic model

Fingerprint

Dive into the research topics of 'Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy'. Together they form a unique fingerprint.

Cite this