Abstract
Retroviruses have two essential activities: reverse transcription and integration. The viral protein integrase (IN) covalently joins the viral cDNA genome to the host DNA. Prototype foamy virus (PFV) IN has become a model of retroviral intasome structure. However, this retroviral IN has not been well-characterized biochemically. Here we compare PFV IN to previously reported HIV-1 IN activities and discover significant differences. PFV IN is able to utilize the divalent cation calcium during strand transfer while HIV-1 IN is not. HIV-1 IN was shown to completely commit to a target DNA within 1 min, while PFV IN is not fully committed after 60 min. These results suggest that PFV IN is more promiscuous compared to HIV-1 IN in terms of divalent cation and target commitment.
| Original language | English |
|---|---|
| Pages (from-to) | 1241-1246 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 503 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 10 2018 |
Keywords
- Divalent cation
- Enzymology
- Integrase
- Prototype foamy virus
- Retrovirus
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