Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

Daniel A. Harki; Jason D. Graci; Jocelyn P. Edathil; Christian Castro; Craig E. Cameron; Blake R. Peterson

doi:10.1002/cbic.200700160

Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

Daniel A. Harki, Jason D. Graci, Jocelyn P. Edathil, Christian Castro, Craig E. Cameron, Blake R. Peterson

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

(Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

Original language	English
Pages (from-to)	1359-1362
Number of pages	4
Journal	ChemBioChem
Volume	8
Issue number	12
DOIs	https://doi.org/10.1002/cbic.200700160
State	Published - Aug 13 2007

Keywords

Antiviral agents
Mutagenesis
Nucleobases
Poliovirus
Polymerases
Universal base

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10.1002/cbic.200700160

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title = "Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase",

abstract = "(Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.",

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AU - Harki, Daniel A.

AU - Graci, Jason D.

AU - Edathil, Jocelyn P.

AU - Castro, Christian

AU - Cameron, Craig E.

AU - Peterson, Blake R.

PY - 2007/8/13

Y1 - 2007/8/13

N2 - (Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

AB - (Figure Presented) Something in the way. Nucleoside analogues that enhance the frequency of viral mutagenesis are a promising new class of antiviral agents. We report that 5-nitroindole ribonucleoside triphosphate (1) can be universally incorporated opposite each native RNA base by the RNA-dependent RNA polymerase (RdRP) from poliovirus, and inhibits this enzyme more potently than the triphosphate metabolite of the antiviral drug, ribavirin.

KW - Antiviral agents

KW - Mutagenesis

KW - Nucleobases

KW - Poliovirus

KW - Polymerases

KW - Universal base

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IS - 12

ER -

Synthesis of a universal 5-nitroindole ribonucleotide and incorporation into RNA by a viral RNA-dependent RNA polymerase

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Keywords

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