The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells

M. Saji; O. Isozaki; T. Tsushima; M. Arai; M. Miyakawa; Y. Ohba; Y. Tsuchiya; T. Sano; K. Shizume

doi:10.1530/acta.0.1190145

The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells

M. Saji
, O. Isozaki
, T. Tsushima
, M. Arai
, M. Miyakawa
, Y. Ohba
, Y. Tsuchiya
, T. Sano
, K. Shizume

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The effect of iodide on growth of rat thyroid cells (FRTL-5) was studied. TSH-stimulated cell growth was inhibited by iodide in a concentration-dependent manner, and an effect of iodide was detected at 10^-6 mmol/l. KClO₄ or 1-methylimidazole-2-thiol blocked the effect of iodide, suggesting that iodide uptake and its organification are required to produce the inhibitory effect of iodide on cell growth. Iodide not only decreased TSH-stimulated cAMP production in FRTL-5 cells but also cell growth induced by cAMP. These observations suggest that iodide inhibits TSH-stimulated growth of the cells by attenuating cAMP production and also by acting on the step(s) distal to cAMP generation. The inhibitory effect of iodide was also seen in growth stimulated by insulin, insulin-like growth factor-I or 12-O-tetradecanoyl phorbol 13-acetate, suggesting multiple sites of action of iodide in the process of growth of FRTL-5 cells.

Original language	English
Pages (from-to)	145-151
Number of pages	7
Journal	Acta Endocrinologica
Volume	119
Issue number	1
DOIs	https://doi.org/10.1530/acta.0.1190145
State	Published - 1988

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title = "The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells",

abstract = "The effect of iodide on growth of rat thyroid cells (FRTL-5) was studied. TSH-stimulated cell growth was inhibited by iodide in a concentration-dependent manner, and an effect of iodide was detected at 10-6 mmol/l. KClO4 or 1-methylimidazole-2-thiol blocked the effect of iodide, suggesting that iodide uptake and its organification are required to produce the inhibitory effect of iodide on cell growth. Iodide not only decreased TSH-stimulated cAMP production in FRTL-5 cells but also cell growth induced by cAMP. These observations suggest that iodide inhibits TSH-stimulated growth of the cells by attenuating cAMP production and also by acting on the step(s) distal to cAMP generation. The inhibitory effect of iodide was also seen in growth stimulated by insulin, insulin-like growth factor-I or 12-O-tetradecanoyl phorbol 13-acetate, suggesting multiple sites of action of iodide in the process of growth of FRTL-5 cells.",

author = "M. Saji and O. Isozaki and T. Tsushima and M. Arai and M. Miyakawa and Y. Ohba and Y. Tsuchiya and T. Sano and K. Shizume",

year = "1988",

doi = "10.1530/acta.0.1190145",

language = "English",

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pages = "145--151",

journal = "Acta Endocrinologica",

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T1 - The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells

AU - Saji, M.

AU - Isozaki, O.

AU - Tsushima, T.

AU - Arai, M.

AU - Miyakawa, M.

AU - Ohba, Y.

AU - Tsuchiya, Y.

AU - Sano, T.

AU - Shizume, K.

PY - 1988

Y1 - 1988

N2 - The effect of iodide on growth of rat thyroid cells (FRTL-5) was studied. TSH-stimulated cell growth was inhibited by iodide in a concentration-dependent manner, and an effect of iodide was detected at 10-6 mmol/l. KClO4 or 1-methylimidazole-2-thiol blocked the effect of iodide, suggesting that iodide uptake and its organification are required to produce the inhibitory effect of iodide on cell growth. Iodide not only decreased TSH-stimulated cAMP production in FRTL-5 cells but also cell growth induced by cAMP. These observations suggest that iodide inhibits TSH-stimulated growth of the cells by attenuating cAMP production and also by acting on the step(s) distal to cAMP generation. The inhibitory effect of iodide was also seen in growth stimulated by insulin, insulin-like growth factor-I or 12-O-tetradecanoyl phorbol 13-acetate, suggesting multiple sites of action of iodide in the process of growth of FRTL-5 cells.

AB - The effect of iodide on growth of rat thyroid cells (FRTL-5) was studied. TSH-stimulated cell growth was inhibited by iodide in a concentration-dependent manner, and an effect of iodide was detected at 10-6 mmol/l. KClO4 or 1-methylimidazole-2-thiol blocked the effect of iodide, suggesting that iodide uptake and its organification are required to produce the inhibitory effect of iodide on cell growth. Iodide not only decreased TSH-stimulated cAMP production in FRTL-5 cells but also cell growth induced by cAMP. These observations suggest that iodide inhibits TSH-stimulated growth of the cells by attenuating cAMP production and also by acting on the step(s) distal to cAMP generation. The inhibitory effect of iodide was also seen in growth stimulated by insulin, insulin-like growth factor-I or 12-O-tetradecanoyl phorbol 13-acetate, suggesting multiple sites of action of iodide in the process of growth of FRTL-5 cells.

UR - https://www.scopus.com/pages/publications/0023790485

U2 - 10.1530/acta.0.1190145

DO - 10.1530/acta.0.1190145

M3 - Article

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AN - SCOPUS:0023790485

SN - 0001-5598

VL - 119

SP - 145

EP - 151

JO - Acta Endocrinologica

JF - Acta Endocrinologica

IS - 1

ER -

The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells

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