Abstract
Murine infection models are needed to develop therapeutics and vaccines to combat the Plasmodium parasites causing malaria. Herein, we describe an easy to perform flow cytometry-based methodology for detecting green fluorescent protein-expressing Plasmodium berghei in the peripheral red blood cells (RBC) of mice. This methodology uses one-step staining and simplified gating strategies to streamline the process of Plasmodium quantification and can detect parasitemia at an earlier time point after infection compared to traditional light microscopy-based techniques.
Original language | English |
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Article number | 106439 |
Journal | Journal of Microbiological Methods |
Volume | 195 |
DOIs | |
State | Published - Apr 2022 |
Keywords
- Flow cytometry
- Green fluorescent protein
- Hoechst 33342
- Malaria
- Parasitemia
- Plasmodium berghei