Protein Semisynthesis

Nam Chu; Philip A. Cole

doi:10.1002/9783527823567.ch11

Protein Semisynthesis

Nam Chu, Philip A. Cole

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

This chapter summarizes major methods and applications of protein synthesis in biochemistry. The use of protein thioesters generated with inteins for use in expressed protein ligation, cysteine modification strategies, and enzyme catalyzed approaches have been highlighted. More recent applications of these methods to analyze the influence of posttranslational modifications on protein structure and function have been emphasized.

Original language	English
Title of host publication	Total Chemical Synthesis of Proteins
Publisher	wiley
Pages	307-326
Number of pages	20
ISBN (Electronic)	9783527823567
ISBN (Print)	9783527346608
DOIs	https://doi.org/10.1002/9783527823567.ch11
State	Published - Jan 1 2021

Keywords

cell signaling
cysteine modification
enzyme-catalyzed EPL
Expressed Protein Ligation (EPL)
Protein semisynthesis

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10.1002/9783527823567.ch11

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title = "Protein Semisynthesis",

abstract = "This chapter summarizes major methods and applications of protein synthesis in biochemistry. The use of protein thioesters generated with inteins for use in expressed protein ligation, cysteine modification strategies, and enzyme catalyzed approaches have been highlighted. More recent applications of these methods to analyze the influence of posttranslational modifications on protein structure and function have been emphasized.",

keywords = "cell signaling, cysteine modification, enzyme-catalyzed EPL, Expressed Protein Ligation (EPL), Protein semisynthesis",

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AB - This chapter summarizes major methods and applications of protein synthesis in biochemistry. The use of protein thioesters generated with inteins for use in expressed protein ligation, cysteine modification strategies, and enzyme catalyzed approaches have been highlighted. More recent applications of these methods to analyze the influence of posttranslational modifications on protein structure and function have been emphasized.

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BT - Total Chemical Synthesis of Proteins

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Protein Semisynthesis

Abstract

Keywords

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