Abstract
Dot-blot analysis is a technique that allows for fast and convenient detection and identification of nucleic acids and proteins. Here, we provide a guide for nucleic acid isolation from eukaryotic cells and sample processing to detect RNA/DNA hybrids. We then provide detailed steps to quantify dot signal intensity. This protocol can be adapted for screening conditions that result in the accumulation of R-loops. For complete details on the use and execution of this protocol, please refer to Smith et al.1
Original language | English |
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Article number | 102857 |
Journal | STAR Protocols |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - Mar 15 2024 |
Keywords
- Cell culture
- Gene Expression
- Molecular Biology